产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human BMP-4 PicoKine ELISA Kit
目录 :
EK0314
规格 :
96 wells/kit, with removable strips.
价格 :
400美元
文章摘录数: 1
参考文献
Stahls P, Lightell D, Moss S, Goldman C, Woods T. Elevated serum bone morphogenetic protein 4 in patients with chronic kidney disease and coronary artery disease. J Cardiovasc Transl Res. 2013;6:232-8 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0314 图像 1
Human BMP-4 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0314
状态 :
Enabled
名称 :
Human BMP-4 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
BMP4
价格 :
400美元
试验范围 :
62.5pg/毫升4000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human BMP4 with <4pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: bone tissue and cell culture supernates. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. BMP4 tissue specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-4. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P12644
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: S293-R408
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<4pg/ml
kit components :
10ng/tube
样品类型 :
bone tissue and cell culture supernates.
应用 :
酶联免疫吸附测定
反应物种 :
人类
前体活性 :
Bovine, Monkey
图像标记 :
Human BMP-4 PicoKine ELISA Kit standard curve
assay procedure step 1 :
4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升
背景 :
Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.
研究种类 :
Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Nervous System Development, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Tgf Beta
同义词 :
骨形态发生蛋白4;BMP-4;骨形态发生蛋白2B;BMP-2B;BMP4;BMP2B, DVR4;
基因全称 :
骨形态发生蛋白4
分子量 :
46555分子量
protein function :
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
subcellular localization :
Secreted, extracellular space, extracellular matrix.
组织特异性 :
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
蛋白名 :
骨形态发生蛋白4
sequence similarities :
Belongs to the TGF-beta family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 196 814 1756 199 823 1888 Standard deviation 12.15 48.02 124.67 12.33 49.38 169.92 CV(%) 6.2% 7.1%% 7.1% 6.2% 6% 9%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 196 183 176 194 187 8.16 4.3% Sample 2 814 793 763 851 805 32.03 3.9% Sample 3 1756 1763 1749 1644 1728 48.74 2.8% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.037 0.083 0.143 0.285 0.523 1.037 1.678 2.412
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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