产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Mouse BMP-4 PicoKine ELISA Kit
目录 :
EK0316
规格 :
96 wells/kit, with removable strips.
价格 :
399美元
文章摘录数: 1
参考文献
Chen W, Yang J, Wu Y, Li L, Li R, Chang Y, et al. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury. Exp Mol Med. 2018;50:36 pubmed 出版商
产品信息
SKU号 :
EK0316
状态 :
Enabled
名称 :
Mouse BMP-4 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
Bmp4
价格 :
399美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Mouse Bmp4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: bone tissue, cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Bmp4 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-4. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P21275
宿主 :
No
免疫原 :
Expression system for standard: CHO; Immunogen sequence: S293-R408
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
bone tissue, cell culture supernates and serum.
应用 :
酶联免疫吸附测定
反应物种 :
小鼠
前体活性 :
Bovine, Monkey
图像标记 :
Mouse BMP-4 PicoKine ELISA Kit standard curve
assay procedure step 1 :
2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升
背景 :
Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.
研究种类 :
Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Nervous System Development, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Tgf Beta
同义词 :
Bone morphogenetic protein 4;BMP-4;Bone morphogenetic protein 2B;BMP-2B;Bmp4;Bmp-4, Dvr-4;
基因全称 :
骨形态发生蛋白4
分子量 :
46497分子量
protein function :
Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
subcellular localization :
Secreted, extracellular space, extracellular matrix.
蛋白名 :
骨形态发生蛋白4
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 53 250 897 54 268 915 Standard deviation 2.65 17.5 52.92 3.07 19.83 53.98 CV(%) 5% 7% 5.9% 5.7% 7.4% 5.9%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 54 54 58 54 1.92 3.5% Sample 2 250 270 229 249 249 14.5 5.8% Sample 3 897 924 940 993 938 35.01 3.7% *number of samples for each test n=16.
样本数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.070 0.104 0.147 0.223 0.394 0.724 1.237 1.900
tmb incubation time :
15-25min
principle :
a单克隆抗体来自大鼠a生物素多克隆抗体来自山羊
更新 :
5/9/19 4:18
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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