产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Rat CNTF PicoKine ELISA Kit
目录 :
EK0324
规格 :
96 wells/kit, with removable strips.
价格 :
333美元
图像
图像 1 :
武汉博士德生物工程有限公司 EK0324 图像 1
Rat CNTF PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0324
状态 :
Enabled
名称 :
Rat CNTF PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
CNTF
价格 :
333美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Rat CNTF with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CNTF tissue specificity: Nervous system.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CNTF. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P20294
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: A2-M200
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
大鼠
图像标记 :
Rat CNTF PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升
背景 :
Ciliary neurotrophic factor(CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid(aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
competitor equivalent skus :
sc 13996 sc 1912 sc 365211 sc 166272 sc 365210 sc 1911 sc 166273 sc 25286 sc 1912 R
研究种类 :
Cell Type Marker, Growth And Development, Neural Stem Cells, Neurology Process, Neuroscience, Stem Cells
同义词 :
Ciliary neurotrophic factor;CNTF;Cntf;
基因全称 :
睫状神经营养因子
分子量 :
22854分子量
protein function :
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
subcellular localization :
细胞质
组织特异性 :
神经系统
蛋白名 :
睫状神经营养因子
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 82 276 868 80 293 809 Standard deviation 4.59 16.83 60.76 5.52 23.14 60.67 CV(%) 6.1%% 6.1% 7% 6.9% 7.9% 7.5%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 75 85 81 80 3.63 4.5% Sample 2 276 262 311 268 279 18.99 6.8% Sample 3 868 1021 877 993 939 68.04 7.2% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000 O.D. 0.024 0.049 0.076 0.123 0.268 0.563 1.338 2.294
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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