产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human EGF PicoKine ELISA Kit
目录 :
EK0325
规格 :
96 wells/kit, with removable strips.
价格 :
320美元
文章摘录数: 3
参考文献
Scalabrino G, Veber D, De Giuseppe R, Roncaroli F. Low levels of cobalamin, epidermal growth factor, and normal prions in multiple sclerosis spinal cord. Neuroscience. 2015;298:293-301 pubmed 出版商
Azuma N, Katada Y, Kitano S, Sekiguchi M, Kitano M, Nishioka A, et al. Rapid decrease in salivary epidermal growth factor levels in patients with Sjögren's syndrome: A 3-year follow-up study. Mod Rheumatol. 2015;25:876-82 pubmed 出版商
Nojiri T, Yoshizato T, Fukami T, Obama H, Yagi H, Yotsumoto F, et al. Clinical significance of amphiregulin and epidermal growth factor in colostrum. Arch Gynecol Obstet. 2012;286:643-7 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0325 图像 1
Human EGF PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0325
状态 :
Enabled
名称 :
Human EGF PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
EGF
价格 :
320美元
试验范围 :
4.7pg/毫升300pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human EGF with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. EGF tissue specificity: Expressed in kidney, salivary gland, cerebrum and prostate.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human EGF. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
E7EVD2
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: N971-R1023
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<1pg/ml
kit components :
1ng/tube
样品类型 :
cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human EGF PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
300pg/毫升, 150pg/毫升, 75pg/毫升, 37.5pg/毫升, 18.8pg/毫升, 9.4pg/毫升, 4.7pg/毫升
背景 :
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
competitor equivalent skus :
sc 1343 sc 275 sc 1342 sc 271090 sc 166779 sc 374255 sc 1341
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction
同义词 :
Pro-epidermal growth factor;EGF;Epidermal growth factor;Urogastrone;EGF;
基因全称 :
Pro-epidermal growth factor
分子量 :
133994分子量
protein function :
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
subcellular localization :
Membrane; Single-pass type I membrane protein.
组织特异性 :
Expressed in kidney, salivary gland, cerebrum and prostate.
蛋白名 :
Pro-epidermal growth factor
sequence similarities :
Contains 9 EGF-like domains.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 11 76 122 10 81 131 Standard deviation 0.71 4.25 9.15 0.82 5.42 12.31 CV(%) 6.5% 5.6% 7.5% 8.2% 6.7% 9.4%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 11 11 12 11 0.43 3.9% Sample 2 76 76 72 68 73 3.31 4.5% Sample 3 122 118 128 133 125 5.71 4.5% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.038 0.129 0.191 0.326 0.623 1.053 1.639 2.634
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
6/10/19 7:06
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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