产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human soluble EGFR PicoKine ELISA Kit
目录 :
EK0327
规格 :
96 wells/kit, with removable strips.
价格 :
320美元
文章摘录数: 1
参考文献
Quaranta M, Divella R, Daniele A, Di Tardo S, Venneri M, Lolli I, et al. Epidermal growth factor receptor serum levels and prognostic value in malignant gliomas. Tumori. 2007;93:275-80 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0327 图像 1
Human EGFR PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0327
状态 :
Enabled
名称 :
Human soluble EGFR PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
表皮生长因子受体
价格 :
320美元
试验范围 :
156pg/毫升10, 000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human EGFR with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and human milk. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. EGFR tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human EGFR. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P00533
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: M1-S645
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<1pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum, plasma(heparin, EDTA) and human milk.
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human EGFR PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
10000pg/毫升, 5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 313pg/毫升, 156pg/毫升
背景 :
The epidermal growth factor receptor(EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family(EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR(ErbB-1), HER2/c-neu(ErbB-2), Her 3(ErbB-3) and Her 4(ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha(TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression(known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
competitor equivalent skus :
sc 101 sc 31155 sc 53274 sc 80543 sc 365829 sc 31156 sc 31157 sc 373746 sc 120 sc 71032 sc 71033 sc 71035 sc 71034 sc 15827 sc 15828 sc 33746 sc 377073 sc 374607 sc 377229 sc 03 sc 367974 sc 514994 sc 514995
研究种类 :
Cancer, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tumor Biomarkers, Tyrosine Kinases
同义词 :
Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1;
基因全称 :
表皮生长因子受体
分子量 :
134277分子量
protein function :
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
subcellular localization :
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
组织特异性 :
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
蛋白名 :
表皮生长因子受体
sequence similarities :
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 289 1634 5526 313 1731 5572 Standard deviation 13.58 81.7 408.92 18.78 102.12 417.9 CV(%) 4.7% 5% 7.4% 6% 5.9% 7.5%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 289 316 285 279 292 14.16 4.8% Sample 2 1634 1690 1737 1686 1686 36.46 2.1% Sample 3 5526 5048 5070 5056 5175 202.8 3.9% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.037 0.105 0.155 0.254 0.463 0.837 1.305 1.773
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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