产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human FAS/Cd95 PicoKine ELISA Kit
目录 :
EK0335
规格 :
96 wells/kit, with removable strips.
价格 :
300美元
文章摘录数: 4
参考文献
Alkhouri N, Alisi A, Okwu V, Matloob A, Ferrari F, Crudele A, et al. Circulating Soluble Fas and Fas Ligand Levels Are Elevated in Children with Nonalcoholic Steatohepatitis. Dig Dis Sci. 2015;60:2353-9 pubmed 出版商
Grygorczuk S, Osada J, Moniuszko A, ÅšwierzbiÅ„ska R, Kondrusik M, Zajkowska J, et al. Increased expression of Fas receptor and Fas ligand in the culture of the peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato. Ticks Tick Borne Dis. 2015;6:189-97 pubmed 出版商
Bollain Y Goytia J, Arellano Rodríguez M, Torres Del Muro F, Daza Benítez L, Muñoz Valle J, Avalos Diaz E, et al. Soluble fas and the -670 polymorphism of fas in lupus nephritis. Int J Nephrol. 2014;2014:780406 pubmed 出版商
Zahorska Markiewicz B, Olszanecka Glinianowicz M, Janowska J, Kocełak P. The effect of weight loss on serum concentrations of FAS and tumour necrosis factor alpha in obese women. Endokrynol Pol. 2008;59:18-22 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0335 图像 1
Human sFAS PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0335
状态 :
Enabled
名称 :
Human FAS/Cd95 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
肿瘤坏死因子受体超家族成员6
价格 :
300美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human Cd95/FAS with <3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd95/FAS tissue specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Soluble FAS. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P25445
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: R17-N173
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<3pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human sFAS PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升
背景 :
Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48-kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.
competitor equivalent skus :
sc 74540 sc 7886 sc 21730 sc 715 sc 8009 sc 1023 sc 1024 sc 714 sc 716 sc 715 G sc 714 G
研究种类 :
Adaptive Immunity, Apoptosis, Autoimmune, Cell Biology, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Lymphocytic Lineage, Tnf Superfamily
同义词 :
Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;FAS;APT1, FAS1, TNFRSF6;
基因全称 :
Tumor necrosis factor receptor superfamily member 6
分子量 :
37732分子量
protein function :
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
subcellular localization :
Isoform 1: Cell membrane; Single-pass type I membrane protein.
组织特异性 :
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
蛋白名 :
Tumor necrosis factor receptor superfamily member 6
sequence similarities :
含1死亡区域
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 68 235 915 63 239 861 Standard deviation 3.6 14.8 44.83 3.33 18.88 54.24 CV(%) 5.3% 7.9% 4.9% 5.3% 7.9% 6.3%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 68 60 71 60 64 4.86 7.5% Sample 2 235 248 233 262 244 11.62 4.7% Sample 3 915 855 754 803 831 59.88 7.2% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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