ELISA/assay

Human FGF4 PicoKine ELISA Kit

EK0345

96 wells/kit, with removable strips.

333美元

Enabled

Human FGF4 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

FGF4

333美元

62.5pg/毫升4000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human FGF4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. FGF4 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF4. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P08620

No

Expression system for standard: E.coli; Immunogen sequence: A31-L206

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human FGF4 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升

Fibroblast growth factor 4 is a protein that in humans is encoded by the FGF4 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. FGF4, a direct target of LEF1 and Wnt signaling, can rescue the arrest of tooth organogenesis in Lef1(-/-) mice.

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Signal Transduction

Fibroblast growth factor 4;FGF-4;Heparin secretory-transforming protein 1;HST;HST-1;HSTF-1;Heparin-binding growth factor 4;HBGF-4;Transforming protein KS3;FGF4;HST, HSTF1, KS3;

成纤维细胞生长因子4

22048分子量

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.

Secreted .

成纤维细胞生长因子4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 132 529 1434 135 579 1324 Standard deviation 9.76 32.26 83.17 12.01 45.74 101.94 CV(%) 7.4% 6.1% 5.8% 8.9% 7.9% 7.7%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 145 143 124 136 8.51 6.2% Sample 2 529 557 529 611 556 33.47 6% Sample 3 1434 1303 1232 1245 1303 79.94 6.1% *number of samples for each test n=16.

Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.053 0.078 0.109 0.153 0.271 0.536 0.824 1.496

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53