产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Rat IGF-1 ELISA Kit PicoKine®
目录 :
EK0377
文章摘录数: 1
参考文献
Talebpour Amiri F, Fadaei Fathabadi F, Mahmoudi Rad M, Piryae A, Ghasemi A, Khalilian A, et al. The effects of insulin-like growth factor-1 gene therapy and cell transplantation on rat acute wound model. Iran Red Crescent Med J. 2014;16:e16323 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0377 图像 1
Rat IGF-1 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0377
状态 :
1
名称 :
Rat IGF-1 ELISA Kit PicoKine®
url key :
rat-igf-1-picokine-trade-elisa-kit-ek0377-boster
产品种类 :
Picokine ELISA Kits
基因名称 :
胰岛素样生长因子1
价格 :
369
试验范围 :
62.5 pg/ml - 4,000 pg/ml
描述 :
Rat IGF-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
short description :
Rat IGF-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P08025
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: G49-A118
储存 :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
交叉活性 :
There is detectable cross-reactivity with IGF-2< 1%.
敏感性 :
<5 pg/ml
kit components :
10 ng/tube
样品类型 :
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
应用细节 :
Dilution ratio of 1:500, concentration in serum is around 142-340ng/ml
应用 :
酶联免疫吸附测定
反应物种 :
大鼠
前体活性 :
仓鼠
images :
/e/k/ek0377.png
图像标记 :
Rat IGF-1 PicoKine ELISA Kit standard curve
样本数据 :
Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.023 0.080 0.141 0.258 0.609 1.133 1.534 1.985
created at :
7/20/14 22:17
updated at :
3/2/22 2:54
meta title :
Rat IGF-1 ELISA Kit Price PicoKine® BosterBio
meta keyword :
Rat IGF-1 ELISA Kit, IGF 1/IGF I ELISA, Bosterbio High Sensitivity
meta description :
Rat IGF-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
rating value :
99.2
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml
背景 :
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
研究种类 :
Cancer,Developmental Biology,Growth Factors,Growth Factors/Hormones,Signal Transduction
同义词 :
胰岛素样生长因子I;IGF-I;促生长因子;胰岛素样生长因子1;胰岛素样生长因子1;
基因全称 :
胰岛素样生长因子I
分子量 :
17079分子量
protein function :
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity).
subcellular localization :
Secreted.
蛋白名 :
胰岛素样生长因子I
sequence similarities :
Belongs to the insulin family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 754 1874 186 764 1861 Standard deviation 9.99 56.55 80.58 11.16 69.52 94.91 CV (%) 5.4% 7.5% 4.3% 6% 9.1% 5.1%
再现性 :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 177 173 194 182 8.04 4.4% Sample 2 754 688 705 708 713 24.45 3.4% Sample 3 1874 1778 1907 1705 1816 79.7 4.3% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
monoclonal antibody from mouse polyclonal antibody from goat specific for IGF-1
normal range citations :
25558384
template :
酶联免疫吸附试剂盒
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits