产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Mouse IGFBP-1 PicoKine ELISA Kit
目录 :
EK0383
规格 :
96 wells/kit, with removable strips.
价格 :
380美元
图像
图像 1 :
武汉博士德生物工程有限公司 EK0383 图像 1
Mouse IGFBP-1 PicoKine ELISA Kit standard curve
图像 2 :
武汉博士德生物工程有限公司 EK0383 图像 2
Mouse IGFBP-1 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0383
状态 :
Enabled
名称 :
Mouse IGFBP-1 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
IGFBP1
价格 :
380美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Mouse IGFBP1 with <3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP1 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP-1. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P47876
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: A26-N272
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<3pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
小鼠
图像标记 :
Mouse IGFBP-1 PicoKine ELISA Kit standard curve Mouse IGFBP-1 PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升
背景 :
IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.
competitor equivalent skus :
sc 6000 sc 55474 sc 25257 sc 13097 sc 6072
研究种类 :
信号转导
同义词 :
Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Igfbp1;Igfbp-1;
基因全称 :
胰岛素样生长因子结合蛋白1
分子量 :
29570分子量
protein function :
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity).
subcellular localization :
Secreted.
蛋白名 :
胰岛素样生长因子结合蛋白1
sequence similarities :
含1 IGFBP N末端区域
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 88 281 865 90 267 783 Standard deviation 6.6 14.77 38.06 7.92 19.49 36.8 CV(%) 7.5% 5.4% 4.4% 8.8% 7.3% 4.7%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 84 87 94 88 3.63 4.1% Sample 2 281 311 260 297 287 18.97 6.6% Sample 3 865 903 817 942 881 46.24 5.2% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.128 0.188 0.240 0.331 0.581 0.941 1.498 2.309
tmb incubation time :
15-25min
principle :
单克隆抗体来自大鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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