ELISA/assay

Mouse IGFBP2 PicoKine ELISA Kit

EK0385

96 wells/kit, with removable strips.

369美元

Enabled

Mouse IGFBP2 PicoKine ELISA Kit

Picokine ELISA Kits

IGFBP2

369美元

125pg/毫升8000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Mouse IGFBP2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP2 tissue specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP2. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P47877

No

Expression system for standard: NSO; Immunogen sequence: E35-Q305

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

小鼠

Mouse IGFBP2 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

8000pg/毫升, 4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升

Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

sc 6002 sc 13096 sc 365368 sc 6001 sc 25285 sc 130070 sc 515134

Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

Insulin-like growth factor-binding protein 2;IBP-2;IGF-binding protein 2;IGFBP-2;mIGFBP-2;Igfbp2;Igfbp-2;

胰岛素样生长因子结合蛋白2

32847分子量

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Secreted.

Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.

胰岛素样生长因子结合蛋白2

含1 IGFBP N末端区域

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV(%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% *number of samples for each test n=16.

Concentration(pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.025 0.123 0.180 0.312 0.546 0.919 1.445 2.036

15-25min

单克隆抗体来自大鼠多克隆抗体来自山羊

4/16/19 4:38