ELISA/assay

Human IGFBP-3 PicoKine ELISA Kit

EK0386

96 wells/kit, with removable strips.

350美元

Enabled

Human IGFBP-3 PicoKine ELISA Kit

Picokine ELISA Kits

胰岛素样生长因子结合蛋白3

350美元

156.2pg/毫升10000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human IGFBP3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP3 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-3. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

B3KPF0

No

Expression system for standard: NSO; Immunogen sequence: A28Q-291

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human IGFBP-3 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

10000pg/毫升, 5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 313pg/毫升, 156pg/毫升

Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

sc 6004 sc 9028 sc 374365 sc 6003 sc 365936 sc 130786 sc 135947

Angiogenesis, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes Associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases

cDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3 ;

胰岛素样生长因子结合蛋白3

8708分子量

胰岛素样生长因子结合蛋白3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 271 1607 5480 253 1639 5548 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8%9 CV(%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 271 288 290 253 275 14.94 5.4% Sample 2 1607 1578 1461 1560 1551 54.87 3.5% Sample 3 5480 5896 5868 5913 5789 179.26 3% *number of samples for each test n=16.

Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.034 0.092 0.160 0.230 0.397 0.691 1.251 1.710

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53