ELISA/assay

Human IGFBP4 PicoKine ELISA Kit

EK0388

96 wells/kit, with removable strips.

369美元

Enabled

Human IGFBP4 PicoKine ELISA Kit

Picokine ELISA Kits

IGFBP4

369美元

500pg/毫升32000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human IGFBP4 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP4 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP4. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P22692

No

NSO, D22-E258

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<20pg/ml

32ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human IGFBP4 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

32000pg/毫升, 16000pg/毫升, 8000pg/毫升, 4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

sc 13092 sc 6005

Angiogenesis, Cardiovascular, Cytoskeleton / Ecm, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway, Structures

胰岛素样生长因子结合蛋白4;IBP-4;IGF结合蛋白4;IGFBP-4;IGFBP4;IBP4;

胰岛素样生长因子结合蛋白4

27934分子量

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Secreted.

胰岛素样生长因子结合蛋白4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 634 3283 15689 630 3596 16628 Standard deviation 29.16 164.15 957.02 40.32 222.95 1330.24 CV(%) 4.6% 5% 6.1% 6.4% 6.2% 8%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 634 669 599 583 621 33.16 5.3% Sample 2 3283 3066 3571 3160 3270 190.05 5.8% Sample 3 15689 15202 16042 14388 15330 620.39 4% *number of samples for each test n=16.

Concentration(pg/ml) 0 16000 32000 2000 4000 8000 16000 32000 O.D. 0.031 0.169 0.200 0.280 0.401 0.660 1.140 1.880

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53