ELISA/assay

Human IL-1 Alpha PicoKine ELISA Kit

EK0389

96 wells/kit, with removable strips.

260美元

Enabled

Human IL-1 Alpha PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

IL1A

260美元

4.7pg/毫升300pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human IL1A with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL1A tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 alpha. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P01583

No

Expression system for standard: E.coli, Immunogen sequence: S113-A271

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<0.5pg/ml

10ng/tube

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

酶联免疫吸附测定

人类

Human IL-1 alpha PicoKine ELISA Kit standard curve

肝素, 乙二胺四乙酸或柠檬酸盐

300pg/毫升, 150pg/毫升, 75pg/毫升, 37.5pg/毫升, 18.8pg/毫升, 9.4pg/毫升, 4.7pg/毫升

Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.

Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology

I型白细胞介素1α;IL-1甲;生血素-1;IL1A;IL1F1;

I型白细胞介素1α

30607分子量

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

I型白细胞介素1α

Belongs to the IL-1 family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 14 52 99 15 49 103 Standard deviation 0.98 2.49 4.35 1.06 2.4 6.07 CV(%) 7% 4.8% 4.4% 7.1% 4.9% 5.9%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 15 15 13 14 0.82 5.8% Sample 2 52 48 50 48 49 1.65 3.3% Sample 3 99 98 112 102 102 5.53 5.4% *number of samples for each test n=16.

Concentration(pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.041 0.097 0.162 0.367 0.582 0.866 1.335 2.133

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53