产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Mouse IL-1 Beta PicoKine ELISA Kit
目录 :
EK0394
规格 :
96 wells/kit, with removable strips.
价格 :
420美元
文章摘录数: 3
参考文献
Huang X, Feng Z, Jiang Y, Li J, Xiang Q, Guo S, et al. VSIG4 mediates transcriptional inhibition of Nlrp3 and Il-1β in macrophages. Sci Adv. 2019;5:eaau7426 pubmed 出版商
Zhang Y, Feng J, Cui J, Yang G, Zhu X. Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box 1 protein. Mol Med Rep. 2018;17:5805-5813 pubmed 出版商
Oh H, Jang J, Li Z, Park S, Yoon K. Effects of umbilical cord serum eye drops in a mouse model of ocular chemical burn. Curr Eye Res. 2012;37:1084-90 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0394 图像 1
Mouse IL-1 beta PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0394
状态 :
Enabled
名称 :
Mouse IL-1 Beta PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits, Cytokine Network
基因名称 :
IL1B
价格 :
420美元
试验范围 :
12.5pg/毫升800pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Mouse Il 1 Beta/IL1B with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 1 Beta/IL1B tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 beta. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P10749
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: V118-S269
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<1pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
小鼠
图像标记 :
Mouse IL-1 beta PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
800pg/毫升, 400pg/毫升, 200pg/毫升, 100pg/毫升, 50pg/毫升, 25pg/毫升, 12.5pg/毫升
背景 :
Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
研究种类 :
Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation
同义词 :
白细胞介素1β;IL-1乙;Il1b;
基因全称 :
白细胞介素1β
分子量 :
30931分子量
protein function :
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
subcellular localization :
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
蛋白名 :
白细胞介素1β
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 33 211 483 36 217 481 Standard deviation 1.74 10.33 34.29 2.19 14.75 35.59 CV(%) 5.3% 4.9% 7.1% 6.1% 6.8% 7.4%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 34 31 32 32 1.11 3.4% Sample 2 211 206 203 208 207 2.91 1.4% Sample 3 483 452 433 430 449 21.1 4.6% *number of samples for each test n=16.
样本数据 :
Concentration(pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.123 0.212 0.336 0.609 1.031 1.798 2.197
tmb incubation time :
15-25min
principle :
单克隆抗体来自大鼠多克隆抗体来自山羊
更新 :
6/10/19 7:38
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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