ELISA/assay

Human IL1R1/Il 1 Ri PicoKine ELISA Kit

EK0395

96 wells/kit, with removable strips.

369美元

Enabled

Human IL1R1/Il 1 Ri PicoKine ELISA Kit

Picokine ELISA Kits

IL1R1

369美元

125pg/毫升8000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Il-1 Ri/IL1R1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il-1 Ri/IL1R1 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL1R1. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P14778

No

Expression system for standard: NSO; Immunogen sequence: D21-T332

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates and serum.

酶联免疫吸附测定

人类

Human IL1R1 PicoKine ELISA Kit standard curve

8000pg/毫升, 4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升

Interleukin 1 receptor, type I(IL1R1), also known as CD121a(Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2(IL1RL2), and interleukin 1 receptor-like 1(IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha(IL1A), interleukin 1 beta(IL1B), and interleukin 1 receptor antagonist(IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.

Atherosclerosis, Cardiovascular, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage, Vascular Inflammation

Interleukin-1 receptor type 1;IL-1R-1;IL-1RT-1;IL-1RT1;CD121 antigen-like family member A;Interleukin-1 receptor alpha;IL-1R-alpha;Interleukin-1 receptor type I;p80;CD121a;Interleukin-1 receptor type 1, membrane form;mIL-1R1;mIL-1RI;Interleukin-1 receptor type 1, soluble form;sIL-1R1;sIL-1RI;IL1R1;IL1R, IL1RA, IL1RT1;

白细胞介素1受体类型1

65402分子量

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.

Membrane ; Single-pass type I membrane protein . Cell membrane . Secreted .

白细胞介素1受体类型1

Belongs to the interleukin-1 receptor family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 188 973 4770 205 1005 4361 Standard deviation 8.46 63.24 195.57 10.86 77.38 248.57 CV(%) 4.5% 6.5% 4.1% 5.3% 7.7% 5.7%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 179 191 173 182 7.15 3.9% Sample 2 973 977 935 964 962 16.42 1.7% Sample 3 4770 4715 5460 5318 5065 327.7 6.4% *number of samples for each test n=16.

Concentration(pg/ml) 0 8000 2000 4000 8000 2000 4000 8000 O.D. 0.088 0.123 0.199 0.307 0.484 0.829 1.454 2.511

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53