ELISA/assay

Human IL-6 PicoKine ELISA Kit

EK0410

96 wells/kit, with removable strips.

250美元

Enabled

Human IL-6 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

白细胞介素-6

250美元

4.69pg/毫升300pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human IL6 with <0.3pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL6 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P05231

No

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<0.3pg/ml

10ng/tube

cell culture supernates, serum and plasma( heparin, EDTA, citrate).

酶联免疫吸附测定

人类

Human IL-6 PicoKine ELISA Kit standard curve

肝素, 乙二胺四乙酸或柠檬酸盐

300pg/毫升, 150pg/毫升, 75pg/毫升, 37.5pg/毫升, 18.75pg/毫升, 9.38pg/毫升, 4.69pg/毫升

The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology

Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6; IFNB2

白细胞介素6

23, 718分子量

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.

白细胞介素6

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 10 38 145 9 37 130 Standard deviation 0.62 1.67 10.73 0.64 1.66 11.18 CV(%) 6.2% 4.4% 7.4% 7.2% 4.5% 8.6%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 10 12 11 10 10 0.82 8.2% Sample 2 38 43 40 42 40 1.92 4.8% Sample 3 145 149 145 150 147 2.27 1.5% *number of samples for each test n=16.

Concentration(pg/ml) 0 4.69 9.38 18.75 37.5 75 150 300 O.D. 0.039 0.107 0.186 0.327 0.616 1.062 1.923 2.420

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

6/5/19 0:54