产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human IL-8 PicoKine ELISA Kit
目录 :
EK0413
规格 :
96 wells/kit, with removable strips.
价格 :
250美元
文章摘录数: 1
参考文献
Cojocaru I, Muşuroi C, Iacob S, Cojocaru M. Investigation of TNF-alpha, IL-6, IL-8 and of procalcitonin in patients with neurologic complications in sepsis. Rom J Intern Med. 2003;41:83-93 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0413 图像 1
Human IL-8 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0413
状态 :
Enabled
名称 :
Human IL-8 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits, Cytokine Network
基因名称 :
CXCL8
价格 :
250美元
试验范围 :
15.6pg/ml-1000pg/ml(cell culture supernates)7.8pg/ml-500pg/ml(serum,plasma)
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human CXCL8 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CXCL8 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-8. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P10145
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: S28-S99
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<1pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human IL-8 PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升
背景 :
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
研究种类 :
Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment
同义词 :
Interleukin-8;IL-8;C-X-C motif chemokine 8;Chemokine (C-X-C motif) ligand 8;Emoctakin;Granulocyte chemotactic protein 1;GCP-1;Monocyte-derived neutrophil chemotactic factor;MDNCF;Monocyte-derived neutrophil-activating peptide;MONAP;Neutrophil-activating protein 1;NAP-1;Protein 3-10C;T-cell chemotactic factor;MDNCF-a;GCP/IL-8 protein IV;IL8/NAP1 form I;Interleukin-8;(Ala-IL-8)77;GCP/IL-8 protein II;IL-8(1-77);IL8/NAP1 form II;MDNCF-b;IL-8(5-77);IL-8(6-77);(Ser-IL-8)72;GCP/IL-8 protein I;IL8/NAP1 form III;Lymphocyte-derived neutrophil-activating factor;LYNAP;MDNCF-c;Neutrophil-activating factor;NAF;IL-8(7-77);GCP/IL-8 protein V;IL8/NAP1 form IV;IL-8(8-77);GCP/IL-8 protein VI;IL8/NAP1 form V;IL-8(9-77);GCP/IL-8 protein III;IL8/NAP1 form VI;CXCL8;IL8;
基因全称 :
白细胞介素8
分子量 :
11098分子量
protein function :
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
subcellular localization :
Secreted.
蛋白名 :
白细胞介素8
sequence similarities :
Belongs to the intercrine alpha (chemokine CxC) family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 16 66 234 16 63 230 Standard deviation 0.72 2.83 14.27 0.8 3.84 17.48 CV(%) 4.5% 4.3% 6.1% 5% 6.1% 7.6%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 16 15 17 16 0.7 4.3% Sample 2 66 67 72 74 69 3.34 4.8% Sample 3 234 249 255 221 239 13.25 5.5% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.106 0.216 0.385 0.817 1.212 1.818 2.243
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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