产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human IL-10 PicoKine ELISA Kit
目录 :
EK0416
规格 :
96 wells/kit, with removable strips.
价格 :
250美元
文章摘录数: 1
参考文献
Chen D, Li Y, Lan S, Pan X, Zhou L, Liang M, et al. [The level and clinical significance of Toll-like receptor 4 in children with severe sepsis]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2011;23:475-7 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0416 图像 1
Human IL-10 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0416
状态 :
Enabled
名称 :
Human IL-10 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits, Cytokine Network
基因名称 :
白细胞介素10
价格 :
250美元
试验范围 :
7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human IL10 with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL10 tissue specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-10. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P22301
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: S19-N178
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<0.5pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma( heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human IL-10 PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.3pg/毫升, 15.6pg/毫升, 7.8pg/毫升
背景 :
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
研究种类 :
Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
同义词 :
白细胞介素10;IL-10;细胞因子合成抑制因子;CSIF;白细胞介素10;
基因全称 :
白细胞介素10
分子量 :
20517分子量
protein function :
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
subcellular localization :
Secreted.
组织特异性 :
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
蛋白名 :
白细胞介素10
sequence similarities :
Belongs to the IL-10 family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 16 53 113 15 54 122 Standard deviation 1.02 2.12 7.34 1.24 3.02 9.88 CV(%) 6.4% 4% 6.5% 8.3% 5.6% 8.1%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 17 15 15 15 0.82 5.4% Sample 2 53 45 47 48 48 2.94 6.1% Sample 3 113 123 106 104 111 7.43 6.6% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.070 0.151 0.187 0.295 0.538 0.858 1.622 2.281
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠单克隆抗体来自小鼠
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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