产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human IL-13 PicoKine ELISA Kit
目录 :
EK0424
规格 :
96 wells/kit, with removable strips.
价格 :
369美元
文章摘录数: 1
参考文献
Doucet J, Zhao A, Fu J, Avrameas A. Development and validation of an ELISA at acidic pH for the quantitative determination of IL-13 in human plasma and serum. Dis Markers. 2013;35:465-74 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0424 图像 1
Human IL-13 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0424
状态 :
Enabled
名称 :
Human IL-13 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits, Cytokine Network
基因名称 :
白细胞介素-13
价格 :
369美元
试验范围 :
15.6pg/毫升1000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human IL13 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL13 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-13. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P35225
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: G21-N132
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human IL-13 PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.3pg/毫升, 15.6pg/毫升
背景 :
Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q.
研究种类 :
Cytokines, Immunology, Innate Immunity, Interleukins
同义词 :
白细胞介素13;IL-13;白细胞介素-13;NC30;
基因全称 :
白细胞介素13
分子量 :
15816分子量
protein function :
Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
subcellular localization :
Secreted.
蛋白名 :
白细胞介素13
sequence similarities :
Belongs to the IL-4/IL-13 family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 34 190 438 32 176 440 Standard deviation 1.93 10.64 24.09 2.36 10.56 29.92 CV(%) 5.7% 5.6% 5.5% 7.4% 6% 6.8%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 30 29 33 31 2.06 6.6% Sample 2 190 180 201 184 188 7.91 4.2% Sample 3 438 424 460 467 447 17.16 3.8% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.057 0.089 0.158 0.294 0.694 1.539 2.522
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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