ELISA/assay

Human IL-17 PicoKine ELISA Kit

EK0430

96 wells/kit, with removable strips.

320美元

Enabled

Human IL-17 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

IL17A

320美元

31.2pg/毫升2000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Il 17/IL17A with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 17/IL17A tissue specificity: Restricted to activated memory T-cells.

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

Q16552

No

Expression system for standard: E.coli; Immunogen sequence: I20-A155

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human IL-17 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升

IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage / Inflamm.

白细胞介素17A;IL-17;IL-17A;细胞毒性T淋巴细胞 抗原8;CTLA-8;IL17A;CTLA8, 白细胞介素-17;

白细胞介素17A

17504分子量

Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

Secreted.

Restricted to activated memory T-cells.

白细胞介素17A

Belongs to the IL-17 family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 54 276 763 52 257 703 Standard deviation 3.88 19.59 34.33 4.52 18.5 37.96 CV(%) 7.2% 7.1% 4.5% 8.7% 7.2% 5.4%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 62 58 57 3.31 5.8% Sample 2 276 273 283 261 273 7.94 2.9% Sample 3 763 768 854 781 791 36.67 4.6% *number of samples for each test n=16.

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.163 0.238 0.383 0.683 1.045 1.663 2.251

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

4/26/19 3:08