ELISA/assay

Human MCP-1 / CCL2 PicoKine ELISA Kit

EK0441

96 wells/kit, with removable strips.

300美元

Enabled

Human MCP-1 / CCL2 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

CCL2

300美元

15.6pg/毫升1000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Mcp1/CCL2 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mcp1/CCL2 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MCP-1. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P13500

No

Expression system for standard: E.coli; Immunogen sequence: Q24-T99

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is cross-reactivity with human Eotaxin, MCP-3<1%.

<1pg/ml

10ng/tube

cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

酶联免疫吸附测定

人类

Human MCP-1 PicoKine ELISA Kit standard curve

肝素, 乙二胺四乙酸或柠檬酸盐

1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升

Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes, Response To Hypoxia, Vascular Inflammation

C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2;

C-C motif chemokine 2

11025分子量

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Secreted.

C-C motif chemokine 2

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 31 159 477 30 148 481 Standard deviation 1.55 8.74 32.91 1.98 9.76 37.51 CV(%) 5% 5.5% 6.9% 6.6% 6.6% 7.8%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 33 37 35 34 2.23 6.5% Sample 2 159 139 156 158 153 8.15 5.3% Sample 3 477 421 423 456 444 23.46 5.2% *number of samples for each test n=16.

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.154 0.244 0.466 1.052 1.298 1.892 2.175

15-25min

多克隆抗体来自山羊多克隆抗体来自山羊

3/14/19 17:14