ELISA/assay

Human CCL8/MCP-2 PicoKine ELISA Kit

EK0442

96 wells/kit, with removable strips.

333美元

Enabled

Human CCL8/MCP-2 PicoKine ELISA Kit

Picokine ELISA Kits

CCL8

333美元

15.6pg/毫升1000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Mcp2/CCL8 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mcp2/CCL8 tissue specificity: Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL8/MCP-2. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P80075

No

Expression system for standard: E.coli; Immunogen sequence: Q24-P99

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human CCL8/MCP-2 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升

Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm.

C-C motif chemokine 8;HC14;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;MCP-2;Small-inducible cytokine A8;MCP-2(6-76);CCL8;MCP2, SCYA10, SCYA8;

C-C motif chemokine 8

11246分子量

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Secreted.

Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.

C-C motif chemokine 8

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 35 152 451 36 154 486 Standard deviation 2.76 9.27 19.84 3.13 11.24 27.7 CV(%) 7.9% 6.1% 4.4% 8.7% 7.3% 5.7%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 34 30 33 2.27 6.8% Sample 2 152 155 149 171 156 8.49 5.4% Sample 3 451 459 443 431 446 10.34 2.3% *number of samples for each test n=16.

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.289 0.442 0.759 1.236 1.612 1.888 2.400

15-25min

多克隆抗体来自山羊多克隆抗体来自山羊

3/7/19 6:53