ELISA/assay

Human CCL7/MCP-3 PicoKine ELISA Kit

EK0443

96 wells/kit, with removable strips.

333美元

Enabled

Human CCL7/MCP-3 PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

CCL7

333美元

15.6pg/毫升1000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Mcp3/CCL7 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mcp3/CCL7 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL7/MCP-3. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P80098

No

Expression system for standard: E.coli; Immunogen sequence: Q24-L99

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human CCL7/MCP-3 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升

Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm.

C-C motif chemokine 7;Monocyte chemoattractant protein 3;Monocyte chemotactic protein 3;MCP-3;NC28;Small-inducible cytokine A7;CCL7;MCP3, SCYA6, SCYA7;

C-C motif chemokine 7

11200分子量

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

Secreted.

C-C motif chemokine 7

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 38 169 505 37 158 504 Standard deviation 2.39 12.67 27.27 2.55 12.79 31.75 CV(%) 8.1% 7.5% 5.4% 6.9% 8.1% 6.3%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 31 31 37 34 3.26 9.5% Sample 2 169 170 168 164 167 2.27 1.3% Sample 3 505 504 427 423 464 39.77 8.5% *number of samples for each test n=16.

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.182 0.323 0.550 1.003 1.418 1.877 2.179

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

5/7/19 5:43