ELISA/assay

Human M-CSF PicoKine ELISA Kit

EK0444

96 wells/kit, with removable strips.

320美元

Enabled

Human M-CSF PicoKine ELISA Kit

Cytokine Network

CSF1

320美元

62.5pg/毫升4000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human M-Csf/CSF1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. M-Csf/CSF1 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human M-CSF. 96wells/kit, with removable strips.

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human M-CSF PicoKine ELISA Kit and many others.

96 wells/kit, with removable strips.

P09603

No

Expression system for standard: E.coli; Immunogen sequence: E33-S190

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine.

酶联免疫吸附测定

人类

Human M-CSF PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升

M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.

Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation

Macrophage colony-stimulating factor 1;CSF-1;M-CSF;MCSF;Lanimostim;Processed macrophage colony-stimulating factor 1;CSF1;

巨噬细胞集落刺激因子1

60179分子量

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Cell membrane ; Single-pass type I membrane protein .

巨噬细胞集落刺激因子1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 152 681 1139 140 716 1136 Standard deviation 8.05 32.68 52.39 9.1 40.09 52.25 CV(%) 5.3% 4.8% 4.6% 6.5% 5.6% 4.6%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 152 153 143 153 150 4.2 2.8% Sample 2 681 697 651 751 695 36.3 5.2% Sample 3 1139 1296 1284 1344 1265 76.54 6% *number of samples for each test n=16.

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.128 0.299 0.537 0.992 1.347 1.803 2.095

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53