ELISA/assay

Mouse M-CSF PicoKine ELISA Kit

EK0445

96 wells/kit, with removable strips.

390美元

Enabled

Mouse M-CSF PicoKine ELISA Kit

Picokine ELISA Kits, Cytokine Network

CCL3

390美元

31.2pg/毫升2000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Mouse Mip 1Alpha/CCL3 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma (EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip 1Alpha/CCL3 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse M-CSF. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

P07141

No

Expression system for standard: E.coli; Immunogen sequence: K33-E262

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates, serum and plasma (EDTA).

酶联免疫吸附测定

小鼠

Mouse M-CSF PicoKine ELISA Kit standard curve

乙二胺四乙酸

2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升

M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation

Macrophage colony-stimulating factor 1;CSF-1;MCSF;Processed macrophage colony-stimulating factor 1;Csf1;Csfm;

巨噬细胞集落刺激因子1

60649分子量

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

Cell membrane ; Single-pass type I membrane protein .

巨噬细胞集落刺激因子1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 40 160 459 36 158 501 Standard deviation 1.68 11.2 21.57 1.9 13.58 30.56 CV(%) 4.2% 7% 4.7% 5.3% 8.6% 6.1%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 41 38 35 38 2.29 6% Sample 2 160 159 164 144 156 7.59 4.8% Sample 3 459 393 401 415 417 25.49 6.1% *number of samples for each test n=16.

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.156 0.239 0.343 0.650 1.278 1.768 2.547

15-25min

单克隆抗体来自大鼠多克隆抗体来自山羊

3/7/19 6:53