ELISA/assay

Mouse MIP-3 Alpha/CCL20 PicoKine ELISA Kit

EK0454

96 wells/kit, with removable strips.

369美元

Enabled

Mouse MIP-3 Alpha/CCL20 PicoKine ELISA Kit

Picokine ELISA Kits

CCL20

369美元

7.8pg/毫升500pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Mouse Mip3A/CCL20 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip3A/CCL20 tissue specificity: Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MIP-3 alpha/CCL20. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

O89093

No

Expression system for standard: E.coli; Immunogen sequence: A28-M97

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<1pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

小鼠

Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.3pg/毫升, 15.6pg/毫升, 7.8pg/毫升

Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

C-C motif chemokine 20;Beta-chemokine exodus-1;CC chemokine LARC;CC chemokine ST38;Liver and activation-regulated chemokine;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20;Ccl20;Larc, Scya20;

C-C motif chemokine 20

10826分子量

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.

Secreted.

Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues.

C-C motif chemokine 20

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 9 55 313 9 57 308 Standard deviation 0.62 3.02 21.28 0.78 3.7 21.56 CV(%) 6.9% 5.5% 6.8% 8.7% 6.5% 7%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 9 9 10 10 9 0.5 5.5% Sample 2 55 56 52 51 53 2.06 3.8% Sample 3 313 301 271 296 295 15.3 5.1% *number of samples for each test n=16.

Concentration(pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.023 0.201 0.394 0.761 1.251 1.852 1.996 2.090

15-25min

单克隆抗体来自大鼠多克隆抗体来自山羊

3/7/19 6:53