ELISA/assay

Human CCL19/MIP-3 Beta PicoKine ELISA Kit

EK0456

96 wells/kit, with removable strips.

369美元

Enabled

Human CCL19/MIP-3 Beta PicoKine ELISA Kit

Picokine ELISA Kits

CCL19

369美元

15.6pg/毫升1000pg/毫升

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

Detect Human Mip-3 Beta/CCL19 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip-3 Beta/CCL19 tissue specificity:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL19/MIP-3 beta. 96wells/kit, with removable strips.

96 wells/kit, with removable strips.

Q6IBD6

No

Expression system for standard: E.coli; Immunogen sequence: G22-S98

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<10pg/ml

10ng/tube

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

Human CCL19/MIP-3 beta PicoKine ELISA Kit standard curve

肝素或乙二胺四乙酸

1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升

Chemokine(C-C motif) ligand 19(CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine(ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

C-C motif chemokine ;CCL19 ;hCG_20476 ;

C-C motif chemokine

10993分子量

Secreted .

C-C motif chemokine 19

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 16.7%7 26.94 1.35 16.4%7 32.23 CV(%) 4.8% 6.7% 6.4% 5% 7.5% 8.1%

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 30 31 29 29 0.82 2.8% Sample 2 234 255 249 222 240 12.9 5.3% Sample 3 421 456 425 465 441 19.07 4.3% *number of samples for each test n=16.

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.066 0.128 0.191 0.395 0.770 1.506 2.265

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

3/7/19 6:53