Mouse CCL19/MIP-3 beta PicoKine ELISA Kit | 武汉博士德生物工程有限公司 EK0457 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

ELISA/assay

产品名称 :

Mouse CCL19/MIP-3 beta PicoKine ELISA Kit

EK0457

规格 :

96 wells/kit, with removable strips.

价格 :

369美元

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

产品信息

SKU号 :

EK0457

状态 :

Enabled

名称 :

Mouse CCL19/MIP-3 beta PicoKine ELISA Kit

目录名称 :

Picokine ELISA Kits

基因名称 :

Ccl19

价格 :

369美元

试验范围 :

15.6pg/毫升1000pg/毫升

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

共轭标签 :

描述 :

Detect Mouse Mip-3 Beta/Ccl19 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Mip-3 Beta/Ccl19 tissue specificity: Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL19/MIP-3 beta. 96wells/kit, with removable strips.

规格 :

96 wells/kit, with removable strips.

UniProt数据库编号 :

O70460

宿主 :

免疫原 :

Expression system for standard: E.coli; Immunogen sequence: G26-S108

储存 :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

交叉活性 :

There is no detectable cross-reactivity with other relevant proteins.

敏感性 :

<10pg/ml

kit components :

10ng/tube

样品类型 :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

应用 :

酶联免疫吸附测定

反应物种 :

小鼠

图像标记 :

Mouse CCL19 PicoKine ELISA Kit standard curve

anticoagulant :

肝素或乙二胺四乙酸

assay procedure step 1 :

1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.3pg/毫升, 15.6pg/毫升

背景 :

Chemokine(C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

研究种类 :

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage / Inflamm., Signal Transduction, Signaling Pathway

同义词 :

C-C motif chemokine 19;Epstein-Barr virus-induced molecule 1 ligand chemokine;EBI1 ligand chemokine;ELC;Small-inducible cytokine A19;Ccl19;Elc, Scya19;

基因全称 :

C-C motif chemokine 19

分子量 :

11911分子量

protein function :

Strongly chemotactic for naive (L-selectinhi) CD4 T- cells and for CD8 T-cells and weakly attractive for resting B- cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

subcellular localization :

Secreted.

组织特异性 :

Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.

蛋白名 :

C-C motif chemokine 19

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV(%) 4% 5.3% 7.7% 5.4% 5.3% 9.6%

再现性 :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 32 28 32 30 1.65 5.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 3 384 401 402 407 398 8.67 2.1% *number of samples for each test n=16.

样品数据 :

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.037 0.073 0.122 0.270 0.644 1.442 2.317

tmb incubation time :

15-25min

更新 :

3/7/19 6:53

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