产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human MMP-1 PicoKine ELISA Kit
目录 :
EK0458
规格 :
96 wells/kit, with removable strips.
价格 :
380美元
文章摘录数: 1
参考文献
Deng C, Ling S, Liu X, Zhao Y, Lv Y. Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia. Exp Ther Med. 2015;9:992-998 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0458 图像 1
Human MMP-1 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0458
状态 :
Enabled
名称 :
Human MMP-1 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
MMP1
价格 :
380美元
试验范围 :
156pg/毫升10000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human MMP1 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MMP1 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-1. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
B4DN15
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: F20-N469
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<20pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma(heparin).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human MMP-1 PicoKine ELISA Kit standard curve
anticoagulant :
肝素
assay procedure step 1 :
10000pg/毫升, 5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 312pg/毫升, 156pg/毫升
背景 :
Matrix metalloproteinase-1(MMP-1) is also known as collagenase. Matrix metalloproteinases(MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1(MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1(interstitial collagenase), MMP9(gelatinase B) and MMP12(macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
研究种类 :
Signal Transduction, Tumor Biomarkers
同义词 :
cDNA FLJ55228, highly similar to Interstitial collagenase (EC 3.4.24.7) ;
基因全称 :
matrix metallopeptidase 1 (interstitial collagenase)
分子量 :
46266分子量
蛋白名 :
间质胶原酶
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 215 1557 4510 235 1708 4065 Standard deviation 13.97 118.33 261.58 19.5 160.55 308.94 CV(%) 6.5% 7.6% 5.8% 8.3% 9.4% 7.6%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 203 207 218 210 6.01 2.8% Sample 2 1557 1530 1547 1360 1498 80.54 5.3% Sample 3 4510 5168 4653 4926 4814 253.07 5.2% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.096 0.130 0.219 0.389 0.661 1.160 2.096
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/26/19 1:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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