产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®
目录 :
EK0525
更多信息或购买 :
文章摘录数: 4
参考文献 |
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Tang H, Li A, Li J, Hou G, Zhang F. [Effect of Ningdong Granule on the levels of IL-12 and TNF-alpha in children patients with Tourette's syndrome]. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014;34:435-8 pubmed
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图像
图像 1 :
Human TNF alpha PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0525
状态 :
1
名称 :
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®
url key :
人类肿瘤坏死因子
产品种类 :
Picokine ELISA Kits
基因名称 :
肿瘤坏死因子
价格 :
369
试验范围 :
15.6 pg/ml - 1,000 pg/ml
描述 :
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
short description :
Human TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Human TNF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P01375
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: V77-L233
储存 :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<1 pg/ml
kit components :
10 ng/tube
样品类型 :
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
应用细节 :
Dilution ratio of 1:1, concentration in serum is around 100pg/ml
应用 :
酶联免疫吸附测定
反应物种 :
人类
images :
/e/k/ek0525_1_8.png
图像标记 :
Human TNF alpha PicoKine ELISA Kit standard curve
样本数据 :
Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.091 0.158 0.197 0.320 0.548 0.897 1.576 2.047
created at :
5/19/14 3:17
updated at :
3/2/22 2:54
meta title :
Human TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine®
meta keyword :
Human TNF alpha ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity
rating value :
97.6
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml
背景 :
Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
研究种类 :
Atherosclerosis,Cancer,Cardiovascular,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Metabolism,Signal Transduction,Tnf Superfamily,Vascular Inflammation
同义词 :
Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;TNF;TNFA, TNFSF2;
基因全称 :
肿瘤坏死因子
分子量 :
25644分子量
protein function :
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918).
subcellular localization :
Cell membrane ; Single-pass type II membrane protein .
蛋白名 :
肿瘤坏死因子
sequence similarities :
Belongs to the tumor necrosis factor family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 82 201 14 75 186 Standard deviation 0.76 5.49 12.66 0.75 5.55 11.9 CV (%) 4.8% 6.7% 7.4% 5.4% 7.4% 6.4%
再现性 :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 15 15 16 15 0.5 3.3% Sample 2 82 91 75 89 84 6.29 7.4% Sample 3 201 204 215 219 209 7.46 3.5% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
normal range citations :
24812899
template :
酶联免疫吸附试剂盒
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits
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