ELISA/assay

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

EK0526

1

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

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Picokine ELISA Kits

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369

15.6 pg/ml - 1,000 pg/ml

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Rat Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Rat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Rat Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

96 wells/kit, with removable strips.

P16599

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

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Rat TNF alpha PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.010 0.031 0.062 0.132 0.203 0.555 0.884 1.838

5/19/14 3:17

3/2/22 2:54

Rat TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine®

Rat TNF Alpha/Tumor necrosis factor ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity

99.6

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1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.

Metabolism,Signal Transduction,Immunology,Cancer,Atherosclerosis,Cardiovascular,Growth Factors/Hormones,Growth Factors,Innate Immunity,Vascular Inflammation,Cytokines,Tnf Superfamily

Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;

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25806分子量

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Cell membrane ; Single-pass type II membrane protein .

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Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 96 280 13 98 306 Standard deviation 0.57 7.39 20.44 0.78 7.54 24.78 CV (%) 4.1% 7.7% 7.3% 6% 7.7% 8.1%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 14 14 14 14 0 0% Sample 2 96 93 92 101 95 3.5 3.6% Sample 3 280 258 274 306 279 17.28 6.1% *number of samples for each test n=16.

15-25min

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24744897

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