ELISA/assay

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

EK0527

1

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®

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Picokine ELISA Kits

肿瘤坏死因子

369

15.6 pg/ml - 1,000 pg/ml

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

96 wells/kit, with removable strips.

P06804

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

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小鼠

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Mouse TNF alpha PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.090 0.156 0.281 0.443 0.723 1.284 1.958

5/18/14 20:17

3/2/22 2:54

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit Price PicoKine®

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit, TNF a ELISA Kit, Bosterbio High Sensitivity

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

99.2

肝素或乙二胺四乙酸

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Metabolism,Signal Transduction,Immunology,Cancer,Atherosclerosis,Cardiovascular,Growth Factors/Hormones,Growth Factors,Innate Immunity,Vascular Inflammation,Cytokines,Tnf Superfamily

Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2;

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25896分子量

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Cell membrane; Single-pass type II membrane protein.

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Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 50 299 16 53 305 Standard deviation 0.82 3.9 22.12 0.91 4.39 24.4 CV (%) 4.6% 7.8% 7.4% 5.7% 8.3% 8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 18 17 19 18 0.7 3.8% Sample 2 50 54 54 47 51 2.94 5.7% Sample 3 299 294 293 285 292 5.01 1.7% *number of samples for each test n=16.

15-25min

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25561799

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