ELISA/assay

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine®

EK0555

1

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine®

人类ccl21

Picokine ELISA Kits

CCL21

369

62.5 pg/ml - 4,000 pg/ml

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

96 wells/kit, with removable strips.

O00585

Expression system for standard: E.coli; Immunogen sequence: S24-P134

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<12 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 500pg/ml

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人类

/e/k/ek0555.png

Human CCL21/6Ckine PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.036 0.067 0.254 0.396 0.970 1.607 2.207

7/16/14 6:17

3/2/22 2:54

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit Price PicoKine®

Human CCL21/6Ckine ELISA Kit, ECL ELISA Kit, Bosterbio High Sensitivity

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

99.1

肝素或乙二胺四乙酸

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Chemokines,Immunology,Innate Immunity

C-C motif chemokine 21;6Ckine;Beta-chemokine exodus-2;Secondary lymphoid-tissue chemokine;SLC;Small-inducible cytokine A21;CCL21;SCYA21;UNQ784/PRO1600;

C-C motif chemokine 21

14646分子量

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Secreted.

Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

C-C motif chemokine 21

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 709 1352 103 706 1475 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 109 108 95 103 5.78 5.6% Sample 2 709 697 706 724 709 9.72 1.3% Sample 3 1352 1517 1447 1423 1434 58.95 4.1% *number of samples for each test n=16.

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

25260647, 23764550

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