Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ | 武汉博士德生物工程有限公司 EK0562 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

ELISA/assay

产品名称 :

Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ

EK0562

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

图像

图像 1 :

Mouse E-Cadherin PicoKine ELISA Kit standard curve

产品信息

SKU号 :

EK0562

状态 :

名称 :

Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ

url key :

小鼠e

产品种类 :

Picokine ELISA Kits

基因名称 :

CDH1

价格 :

430

试验范围 :

156 pg/ml - 10,000 pg/ml

描述 :

Mouse E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

short description :

Mouse E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

规格 :

96 wells/kit, with removable strips.

UniProt数据库编号 :

P09803

免疫原 :

Expression system for standard: NS0; Immunogen sequence: D157-V709

储存 :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

交叉活性 :

There is no detectable cross-reactivity with other relevant proteins.

敏感性 :

<15 pg/ml

kit components :

10 ng/tube

样品类型 :

cell culture supernatants, serum and plasma (heparin).

应用细节 :

Dilution ratio of 1:200, concentration in serum is 262-339ng/ml

应用 :

酶联免疫吸附测定

反应物种 :

小鼠

images :

/e/k/ek0562.png

图像标记 :

Mouse E-Cadherin PicoKine ELISA Kit standard curve

样本数据 :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.050 0.132 0.204 0.374 0.645 1.118 1.865 2.342

created at :

5/18/14 6:17

updated at :

3/2/22 2:54

meta title :

Mouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit Price PicoKine¬Æ

meta keyword :

Mouse E-Cadherin ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Mouse E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

rating value :

98.5

anticoagulant :

肝素

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml

背景 :

E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

研究种类 :

Cadherins,Calcium Binding Proteins,Calcium Signaling,Cancer,Cell Adhesion,Cytoskeleton / Ecm,Invasion/Microenvironment,Signal Transduction,Signaling Pathway

同义词 :

Cadherin-1;ARC-1;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;Cdh1;

基因全称 :

钙粘蛋白1

分子量 :

98256分子量

protein function :

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).

subcellular localization :

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity).

组织特异性 :

Non-neural epithelial tissues.

蛋白名 :

钙粘蛋白1

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3%

再现性 :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

单克隆抗体来自大鼠多克隆抗体来自山羊

template :

酶联免疫吸附试剂盒

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

公司信息