产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Mouse VEGFR2/KDR ELISA Kit PicoKine®
目录 :
EK0590
更多信息或购买 :
图像
图像 1 :
Mouse VEGFR2 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0590
状态 :
1
名称 :
Mouse VEGFR2/KDR ELISA Kit PicoKine®
url key :
小鼠vegfr2
产品种类 :
Picokine ELISA Kits
基因名称 :
激酶插入区受体
价格 :
399
试验范围 :
62.5 pg/ml - 4,000 pg/ml
描述 :
Mouse VEGFR2/KDR ELISA Kit PicoKine (96 Tests). Quantitate Mouse Kdr in cell culture supernatants and serum. Sensitivity: 2pg/ml.
short description :
Mouse VEGFR2/KDR ELISA Kit PicoKine (96 Tests). Quantitate Mouse Kdr in cell culture supernatants and serum. Sensitivity: 2pg/ml.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P35918
免疫原 :
Expression system for standard: NS0; Immunogen sequence: A20-E762
储存 :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<2 pg/ml
kit components :
10 ng/tube
样品类型 :
cell culture supernatants and serum.
应用细节 :
Dilution ratio of 1:20, concentration in serum is 20-40ng/ml
应用 :
酶联免疫吸附测定
反应物种 :
小鼠
images :
/e/k/ek0590_1.png
图像标记 :
Mouse VEGFR2 PicoKine ELISA Kit standard curve
样本数据 :
Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.105 0.160 0.287 0.514 0.903 1.463 2.077
created at :
7/15/14 23:17
updated at :
3/2/22 2:54
meta title :
Mouse VEGFR2/KDR ELISA Kit Price PicoKine® BosterBio
meta keyword :
小鼠VEGFR2/激酶插入区受体酶联免疫吸附试剂盒, 血管内皮生长因子受体2酶联免疫吸附试剂盒
meta description :
Mouse VEGFR2/KDR ELISA Kit PicoKine (96 Tests). Quantitate Mouse Kdr in cell culture supernatants and serum. Sensitivity: 2pg/ml.
rating value :
98.6
assay procedure step 1 :
4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml
背景 :
Vascular endothelial growth factor receptor 2 (VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
研究种类 :
Metabolism Processes,Microbiology,Oncoproteins,Oncoproteins/Suppressors,Pathways And Processes,Protein Phosphorylation,Receptor Tyrosine Kinases,Response To Hypoxia,Signal Transduction,Stem Cells,Surface Molecules,Tyrosine Kinases
同义词 :
Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase NYK;Protein-tyrosine kinase receptor flk-1;CD309;Kdr;Flk-1, Flk1;
基因全称 :
血管内皮生长因子受体2
分子量 :
152517分子量
protein function :
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC- mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
subcellular localization :
Cell junction . Endoplasmic reticulum . Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (By similarity). Localized with RAP1A at cell-cell junctions.
组织特异性 :
Expressed in endothelial cells (at protein level). Detected in embryonic endothelial cells, as well as hematopoietic stem and progenitor cells. Detected in vascular endothelium. Expressed at high levels in adult heart, lung, kidney, brain and skeletal muscle, but is also expressed at lower levels in most other adult tissues.
蛋白名 :
血管内皮生长因子受体2
sequence similarities :
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 585 1309 133 543 1323 Standard deviation 6.83 36.27 60.21 9.31 39.09 63.5 CV (%) 4.8%% 6.2% 4.6% 7% 7.2% 4.8%
再现性 :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 118 135 128 128 6.75 5.2% Sample 2 585 644 654 632 628 26.43 4.2% Sample 3 1309 1087 1250 1292 1234 87.82 7.1% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
单克隆抗体来自大鼠多克隆抗体来自山羊
template :
酶联免疫吸附试剂盒
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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