ELISA/assay

Rat MMP-2 ELISA Kit PicoKine®

EK0639

1

Rat MMP-2 ELISA Kit PicoKine®

大鼠基质金属蛋白酶

Picokine ELISA Kits

MMP2

400

625 pg/ml - 40,000 pg/ml

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P33436

Expression system for standard: NS0; Immunogen sequence: A30-C662

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

40 ng/tube

cell culture supernatants, serum and plasma (heparin).

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大鼠

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Rat MMP-2 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.131 0.168 0.213 0.332 0.585 0.925 1.498 2.082

7/15/14 9:17

3/2/22 2:54

Rat MMP-2 ELISA Kit Price PicoKine® BosterBio

Rat MMP-2 ELISA Kit, MMP2/MONA ELISA, Bosterbio High Sensitivity

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

98.9

肝素

40,000 pg/ml, 20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Extracellular Matrix,Invasion/Microenvironment,Metabolism,Metabolism Processes,Metalloprotease,Pathways And Processes,Proteolysis / Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Tumor Biomarkers

72kDa的IV型胶原酶;3.4。 24.24;72kDa的明胶酶;明胶酶a;基质金属蛋白酶2;基质金属蛋白酶-2;PEX;Mmp2;

72kDa的IV型胶原酶

74149分子量

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).

Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).

72kDa的IV型胶原酶

Belongs to the peptidase M10A family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1093 1090 975 1117 1068 55.12 5.1% Sample 2 4492 3849 4402 4495 4309 268.48 6.2% Sample 3 23073 23113 22919 26456 23890 1483.1 6.2% *number of samples for each test n=16.

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

24033225

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