ELISA/assay

Human CXCL4/PF4 ELISA Kit PicoKine®

EK0726

1

Human CXCL4/PF4 ELISA Kit PicoKine®

人类cxcl4

Picokine ELISA Kits

PF4

369

156 pg/ml - 10,000 pg/ml

Human CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Human PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Human CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Human PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

96 wells/kit, with removable strips.

P02776

Expression system for standard: E.coli; Immunogen sequence: E32-S101

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<15 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

酶联免疫吸附测定

人类

/e/k/ek0726_2.png

Human CXCL4/PF4 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.081 0.127 0.196 0.264 0.381 0.661 1.292 1.991

3/28/16 6:50

3/2/22 2:55

Human CXCL4/PF4 ELISA Kit Price PicoKine® BosterBio

Human CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Human PF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

100

肝素或乙二胺四乙酸

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Immunology,Atherosclerosis,Cardiovascular,Innate Immunity,Macrophage / Inflamm.,Vascular Inflammation,Platelets,Chemokines

Platelet factor 4;PF-4;C-X-C motif chemokine 4;Iroplact;Oncostatin-A;Platelet factor 4, short form;PF4;CXCL4, SCYB4;

血小板因子

10845分子量

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.

Secreted.

血小板因子

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 355 1700 4798 337 1547 5118 Standard deviation 24.85 79.9 249.49 24.26 91.27 348.02 CV (%) 7% 4.7% 5.2% 7.2% 5.9% 6.8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 355 302 353 316 331 23.04 6.9% Sample 2 1700 1790 1823 1661 1743 65.53 3.7% Sample 3 4798 4682 4616 4446 4635 127.33 2.7% *number of samples for each test n=16.

15-25min

a单克隆抗体来自小鼠a生物素多克隆抗体来自山羊

酶联免疫吸附试剂盒