ELISA/assay

Mouse CXCL4/PF4 ELISA Kit PicoKine®

EK0727

1

Mouse CXCL4/PF4 ELISA Kit PicoKine®

小鼠cxcl4

Picokine ELISA Kits

CXCL4

369

15.6 pg/ml - 1,000 pg/ml

Mouse CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Pf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Mouse CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Pf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

96 wells/kit, with removable strips.

Q9Z126

Expression system for standard: E.coli; Immunogen sequence: V30-S105

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:20000, concentration in serum is 10-40ug/ml

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小鼠

/e/k/ek0727_1.png

Mouse CXCL4/PF4 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.030 0.068 0.109 0.183 0.384 0.872 1.829 2.737

7/12/14 18:17

3/2/22 2:54

Mouse CXCL4/PF4 ELISA Kit Price PicoKine® BosterBio

Mouse CXCL4/PF4 ELISA Kit, Bosterbio High Sensitivity

Mouse CXCL4/PF4 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Pf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

99.2

肝素或乙二胺四乙酸

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Atherosclerosis,Cardiovascular,Chemokines,Immunology,Innate Immunity,Macrophage / Inflamm.,Platelets,Vascular Inflammation

Platelet factor 4;PF-4;C-X-C motif chemokine 4;Pf4;Cxcl4, Scyb4;

血小板因子

11243分子量

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).

Secreted.

血小板因子

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 263 425 33 253 425 Standard deviation 1.69 11.04 28.05 2.27 17.28 30.6 CV (%) 5.3% 4.2% 6.6% 6.9% 6% 7.2%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 32 34 33 32 0.82 2.5% Sample 2 263 259 241 228 247 14.09 5.7% Sample 3 425 469 430 397 430 25.66 5.9% *number of samples for each test n=16.

15-25min

单克隆抗体来自大鼠多克隆抗体来自山羊

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