ELISA/assay

Mouse CSF1R/M-CSFR ELISA Kit PicoKine®

EK0808

1

Mouse CSF1R/M-CSFR ELISA Kit PicoKine®

小鼠csf1r

Picokine ELISA Kits

CSF1R

369

62.5 pg/ml - 4,000 pg/ml

Mouse CSF1R/M-CSFR ELISA Kit PicoKine (96 Tests). Quantitate Mouse Csf1r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Mouse CSF1R/M-CSFR ELISA Kit PicoKine (96 Tests). Quantitate Mouse Csf1r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P09581

Expression system for standard: NS0; Immunogen sequence: A20-S511

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1000, concentration in serum is 571-947ng/ml

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小鼠

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Mouse CSF1R/M-CSFR PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992

5/12/14 3:17

3/2/22 2:54

Mouse CSF1R/M-CSFR ELISA Kit Price PicoKine® BosterBio

小鼠CSF1R/M-CSFR酶联免疫吸附试剂盒, MCSF受体酶联免疫吸附测定

99

肝素或乙二胺四乙酸

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

CSF1R (Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Angiogenesis,Cardiovascular,Cytokines,Immunology,Innate Immunity,Macrophage / Inflamm.,Protein Phosphorylation,Ser / Thr Kinases,Signal Transduction

Macrophage colony-stimulating factor 1 receptor;CSF-1 receptor;CSF-1-R;CSF-1R;M-CSF-R;2.7.10.1;Proto-oncogene c-Fms;CD115;Csf1r;Csfmr, Fms;

巨噬细胞集落刺激因子1受体

109179分子量

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP- 1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.

Cell membrane ; Single-pass type I membrane protein . The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

Widely expressed.

巨噬细胞集落刺激因子1受体

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 121 107 111 5.95 5.3% Sample 2 398 456 393 395 410 26.32 6.4% Sample 3 1799 1871 1812 1936 1854 54.31 2.9% *number of samples for each test n=16.

15-25min

单克隆抗体来自大鼠多克隆抗体来自山羊

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