Human Diablo/SMAC ELISA Kit PicoKine¬Æ | 武汉博士德生物工程有限公司 EK0838 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

ELISA/assay

产品名称 :

Human Diablo/SMAC ELISA Kit PicoKine¬Æ

EK0838

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

图像

图像 1 :

Human Diablo/SMAC PicoKine ELISA Kit standard curve

产品信息

SKU号 :

EK0838

状态 :

名称 :

Human Diablo/SMAC ELISA Kit PicoKine¬Æ

url key :

human-diablo-smac-picokine-trade-elisa-kit-ek0838-boster

产品种类 :

Picokine ELISA Kits

基因名称 :

DIABLO

价格 :

369

试验范围 :

31.2 pg/ml - 2,000 pg/ml

描述 :

Human Diablo/SMAC ELISA Kit PicoKine (96 Tests). Quantitate Human DIABLO in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human Diablo/SMAC ELISA Kit PicoKine (96 Tests). Quantitate Human DIABLO in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

规格 :

96 wells/kit, with removable strips.

UniProt数据库编号 :

Q9NR28

免疫原 :

Expression system for standard: E.coli; Immunogen sequence: A56-D239

储存 :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

交叉活性 :

There is no detectable cross-reactivity with other relevant proteins.

敏感性 :

<10 pg/ml

kit components :

10 ng/tube

样品类型 :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

应用细节 :

Dilution ratio of 1:1, concentration in serum is around 60pg/ml

应用 :

酶联免疫吸附测定

反应物种 :

人类

images :

/antibody/ek0838-2-ELISA-human-diablo-smac-picokine-elisa-kit.jpg

图像标记 :

Human Diablo/SMAC PicoKine ELISA Kit standard curve

样本数据 :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.049 0.096 0.193 0.358 0.773 1.338 2.294

created at :

5/10/14 23:17

updated at :

3/2/22 2:54

meta title :

Human Diablo/SMAC ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Diablo/SMAC ELISA Kit, Bosterbio High Sensitivity

meta description :

Human Diablo/SMAC ELISA Kit PicoKine (96 Tests). Quantitate Human DIABLO in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

96.5

anticoagulant :

肝素或乙二胺四乙酸

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

背景 :

Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases (SMAC), is a protein that in humans is encoded by the DIABLO gene (direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A (MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.

competitor equivalent skus :

sc 15680 sc 15678

研究种类 :

Apoptosis,Cancer,Cell Biology,Cell Death,Invasion/Microenvironment,Metabolism,Metabolism Processes,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways And Processes,Receptors

同义词 :

Diablo homolog, mitochondrial;Direct IAP-binding protein with low pI;Second mitochondria-derived activator of caspase;Smac;DIABLO;SMAC;

基因全称 :

Diablo homolog, mitochondrial

分子量 :

27131分子量

protein function :

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

subcellular localization :

Mitochondrion . Released into the cytosol when cells undergo apoptosis.

组织特异性 :

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

蛋白名 :

Diablo homolog, mitochondrial

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 221 865 53 229 932 Standard deviation 4.05 17.01 38.83 4.61 20.15 48.46 CV (%) 7.5% 7.7% 4.2% 8.7% 8.8% 5.2%

再现性 :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 55 55 54 0.5 0.9% Sample 2 221 218 222 235 224 6.51 2.9% Sample 3 865 888 885 882 880 8.91 1% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

单克隆抗体来自小鼠多克隆抗体来自山羊

template :

酶联免疫吸附试剂盒

更多信息或购买 :

武汉博士德生物工程有限公司产品网页

公司信息