ELISA/assay

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine®

EK0868

96 wells/kit, with removable strips.

399美元

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine®

96 wells/kit, with removable strips.

399美元

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine® (96 Tests). Quantitate Human DKK4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

人类

酶联免疫吸附测定

DKK4

Q9UBT3

Expression system for standard: NS0; Immunogen sequence: L19-L224

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

<10 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA)

a单克隆抗体来自小鼠, a生物素多克隆抗体来自山羊

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

dickkopf WNT signaling pathway inhibitor 4

Dickkopf-related protein 4; Dickkopf-4; Dkk-4; hDkk-4; Dickkopf-related protein 4 short form; DKK4

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

Secreted.

Expressed in cerebellum, T-cells, esophagus and lung.

DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Secreted, Signaling Pathways, Stem Cells