产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human HSP27 / Heat Shock Protein 27 PicoKine ELISA Kit
目录 :
EK0881
规格 :
96 wells/kit, with removable strips.
价格 :
380美元
文章摘录数: 1
参考文献
Jan Ankersmit H, Nickl S, Hoeltl E, Toepker M, Lambers C, Mitterbauer A, et al. Increased serum levels of HSP27 as a marker for incipient chronic obstructive pulmonary disease in young smokers. Respiration. 2012;83:391-9 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0881 图像 1
Human HSP27 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0881
状态 :
Enabled
名称 :
Human HSP27 / Heat Shock Protein 27 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
HSPB1
价格 :
380美元
试验范围 :
62.5pg/毫升4000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human Hsp27/HSPB1 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Hsp27/HSPB1 tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HSP27. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P04792
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: T2-K205
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<5pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma(heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human HSP27 PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升
背景 :
Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.
研究种类 :
Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers
同义词 :
Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28;
基因全称 :
热休克蛋白乙1
分子量 :
22783分子量
protein function :
Involved in stress resistance and actin organization.
subcellular localization :
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
组织特异性 :
Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
蛋白名 :
热休克蛋白乙1
sequence similarities :
Belongs to the small heat shock protein (HSP20) family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 82 492 1757 81 468 1729 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 CV(%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 88 78 91 84 5.06 6% Sample 2 492 526 485 501 501 15.5 3% Sample 3 1757 1541 1607 1549 1613 86.67 5.3% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.099 0.119 0.173 0.253 0.384 0.697 1.127 1.796
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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