产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human Endostatin ELISA Kit PicoKine®
目录 :
EK0886
文章摘录数: 4
参考文献
Salza R, Oudart J, Ramont L, Maquart F, Bakchine S, Thoannes H, et al. Endostatin level in cerebrospinal fluid of patients with Alzheimer's disease. J Alzheimers Dis. 2015;44:1253-61 pubmed 出版商
Ã…kerfeldt T, Gunningberg L, Swenne C, Ronquist G, Larsson A. Elective orthopedic and cardiopulmonary bypass surgery causes a reduction in serum endostatin levels. Eur J Med Res. 2014;19:61 pubmed 出版商
Hu M, Hu Y, Zhang H, Jia W, Qian Z, Yang Y, et al. Clinical significance of serum and tumor tissue endostatin evaluation in operable non-small cell lung cancer. Biomed Rep. 2014;2:898-904 pubmed
Liu Y, Zhou Q, Lu Y, Zhu W, Wang Y, Zhang J, et al. [Serum levels of endostatin and VEGF in non-small cell lung cancer patients and the relationship with the clinical pathophysiological characteristics]. Zhongguo Fei Ai Za Zhi. 2004;7:50-4 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0886 图像 1
Human Endostatin PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0886
状态 :
1
名称 :
Human Endostatin ELISA Kit PicoKine®
url key :
人类内皮抑素
产品种类 :
Picokine ELISA Kits
基因名称 :
COL18A1
价格 :
369
试验范围 :
156 pg/ml - 10,000 pg/ml
描述 :
Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.
short description :
Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P39060
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: H1154-S1335
储存 :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10 pg/ml
kit components :
20 ng/tube
样品类型 :
cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
应用细节 :
Dilution ratio of 1:20, concentration in serum is 30-50ng/ml
应用 :
酶联免疫吸附测定
反应物种 :
人类
images :
/antibody/ek0886-2-ELISA-human-endostatin-picokine-elisa-kit.jpg
图像标记 :
Human Endostatin PicoKine ELISA Kit standard curve
样本数据 :
Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.294 0.596 0.980 1.323 1.555 1.695 1.916
created at :
7/8/14 9:17
updated at :
3/2/22 2:54
meta title :
Human Endostatin ELISA Kit Price PicoKine® BosterBio
meta keyword :
人类内皮抑素酶联免疫吸附试剂盒, 甲1型胶原十八
meta description :
Human Endostatin ELISA Kit PicoKine (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.
rating value :
97.6
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml
背景 :
Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
competitor equivalent skus :
sc 16651 sc 25720 sc 32720 sc 514355
研究种类 :
Angiogenesis,Cancer,Cardiovascular,Collagen,Cytoskeleton / Ecm,Ecm Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction
同义词 :
胶原甲1(XVIII)链;内皮抑素;COL18A1;
基因全称 :
胶原甲1(XVIII)链
分子量 :
178188分子量
protein function :
COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
subcellular localization :
Secreted, extracellular space, extracellular matrix .
组织特异性 :
Present in multiple organs with highest levels in liver, lung and kidney.
蛋白名 :
胶原甲1(XVIII)链
sequence similarities :
Belongs to the multiplexin collagen family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1523 5144 251 1462 5243 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1%
再现性 :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 238 232 204 230 16.06 6.9% Sample 2 1523 1309 1362 1479 1418 86.25 6% Sample 3 5144 5141 5851 5778 5478 336.99 6.1% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
normal range citations :
25408220, 25380724, 25279166, 21211193
template :
酶联免疫吸附试剂盒
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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