ELISA/assay

Human P53 ELISA Kit PicoKine®

EK0895

1

Human P53 ELISA Kit PicoKine®

人类p53

Picokine ELISA Kits

TP53

420

156 pg/ml - 10,000 pg/ml

Human P53 ELISA Kit PicoKine (96 Tests). Quantitate Human TP53 in cell lysates. Sensitivity: 10pg/ml.

Human P53 ELISA Kit PicoKine (96 Tests). Quantitate Human TP53 in cell lysates. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

K7PPA8

Expression system for standard: NS0; Immunogen sequence: M1-D393

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell lysates.

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

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人类

/e/k/ek0895.png

Human P53 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.108 0.172 0.296 0.536 1.062 1.725 2.313

5/10/14 9:17

3/2/22 2:54

Human P53 ELISA Kit Price PicoKine® BosterBio

Human P53 ELISA Kit, Antigen NY CO 13 ELISA, Bosterbio High Sensitivity

Human P53 ELISA Kit PicoKine (96 Tests). Quantitate Human TP53 in cell lysates. Sensitivity: 10pg/ml.

99.3

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Apoptosis,Cell Biology,Intracellular,P53 Pathway

Cellular tumor antigen p53 ;TP53 ;

Cellular tumor antigen p53

43653分子量

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

Cytoplasm. Nucleus.

Cellular tumor antigen p53

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 275 1540 5055 302 1396 5437 Standard deviation 16.5 72.38 394.29 20.83 71.19 467.58 CV (%) 6% 4.7% 7.8% 6.9% 8.6% 8.6%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 275 249 256 233 253 15.07 5.9% Sample 2 1540 1645 1702 1738 1656 74.86 4.5% Sample 3 5055 4442 4533 4238 4567 301.31 6.5% *number of samples for each test n=16.

15-25min

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9098910

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