产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®
目录 :
EK0898
图像
图像 1 :
武汉博士德生物工程有限公司 EK0898 图像 1
Human Tryptase PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0898
状态 :
1
名称 :
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®
url key :
human-tryptase-tpsab1-b2-picokine-trade-elisa-kit-ek0898-boster
产品种类 :
Picokine ELISA Kits
基因名称 :
TPSB1/TPSB2
价格 :
369
试验范围 :
156 pg/ml - 10,000 pg/ml
描述 :
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine (96 Tests). Quantitate Human TPSB1/TPSB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
short description :
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine (96 Tests). Quantitate Human TPSB1/TPSB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
Q15661/P20231
免疫原 :
Expression system for standard: NS0; Immunogen sequence: M1-V275
储存 :
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<15 pg/ml
kit components :
10 ng/tube
样品类型 :
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
应用细节 :
Dilution ratio of 1:1, concentration in serum is 2.5-5ng/ml
应用 :
酶联免疫吸附测定
反应物种 :
人类
images :
/e/k/ek0898_2.png
图像标记 :
Human Tryptase PicoKine ELISA Kit standard curve
样本数据 :
Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.113 0.212 0.301 0.459 0.714 1.236 1.861
created at :
3/29/16 6:15
updated at :
3/2/22 2:55
meta title :
Human Tryptase/TPSAB1,B2 ELISA Kit Price PicoKine® BosterBio
rating value :
97.2
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml
背景 :
Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
研究种类 :
Cell Biology,Immunology,Innate Immunity,Interspecies Interaction,Microbiology,Proteolysis / Ubiquitin,Proteolytic Enzymes,Secreted Molecules
同义词 :
Tryptase beta-2;Tryptase-2;3.4.21.59;Tryptase II;TPSB2;TPS2;
基因全称 :
Tryptase beta-2
分子量 :
30515分子量
protein function :
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
subcellular localization :
Secreted. Released from the secretory granules upon mast cell activation.
组织特异性 :
TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).
蛋白名 :
Tryptase alpha/beta-1/beta-2
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 276 1887 5608 283 2054 5485 Standard deviation 18.49 143.41 420.6 23.48 191.02 488.16 CV (%) 6.7% 7.6% 7.5% 8.3% 9.3% 8.9%
再现性 :
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 276 255 284 276 272 10.75 3.9% Sample 2 1887 1991 1959 1839 1919 59.59 3.1% Sample 3 5608 5276 5559 5824 5566 195.24 3.5% *number of samples for each test n=16.
tmb incubation time :
15-25min
principle :
多克隆抗体来自山羊多克隆抗体来自山羊
template :
酶联免疫吸附试剂盒
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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