产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Dog Canine IL-29 PicoKine ELISA Kit
目录 :
EK0964-CN
规格 :
96 wells/kit, with removable strips.
价格 :
333美元
文章摘录数: 1
参考文献
Wang F, Xu L, Feng X, Guo D, Tan W, Zhang M. Interleukin-29 modulates proinflammatory cytokine production in synovial inflammation of rheumatoid arthritis. Arthritis Res Ther. 2012;14:R228 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0964-CN 图像 1
Dog IL-29 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0964-CN
状态 :
Enabled
名称 :
Dog Canine IL-29 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
IFNL1
价格 :
333美元
试验范围 :
62.5pg/毫升4000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Dog Il 29/IFNL1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 29/IFNL1 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Dog Caninea IL-29. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
Q8IU54
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: G20-T200
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and plasma(heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
图像标记 :
Dog IL-29 PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升
背景 :
Interferon lambda-1, also called Interleukin-29(IL-29) is a protein that in Dog Canineas is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
研究种类 :
Cytokines, Immunology, Innate Immunity, Interleukins
同义词 :
Interferon lambda-1;IFN-lambda-1;Cytokine Zcyto21;Interleukin-29;IL-29;IFNL1;IL29, ZCYTO21;
基因全称 :
Interferon lambda-1
分子量 :
21898分子量
protein function :
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
subcellular localization :
Secreted.
蛋白名 :
Interferon lambda-1
sequence similarities :
Belongs to the lambda interferon family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 90 415 1566 103 448 1895 Standard deviation 3.78 22.00 72.04 4.94 28.67 138.34 CV(%) 4.2% 5.3% 4.6% 4.8% 6.4% 7.3%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 91 83 91 88 3.34 3.7% Sample 2 415 360 421 388 396 24.21 6.1% Sample 3 1566 1471 1612 1562 1552 51.12 3.2% *number of samples for each test n=16.
样本数据 :
Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.072 0.159 0.208 0.303 0.535 0.905 1.319 2.280
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits