ELISA/assay

Human CDNF ELISA Kit PicoKine®

EK1656

96 wells/kit, with removable strips.

399美元

Human CDNF ELISA Kit PicoKine®

96 wells/kit, with removable strips.

399美元

Human CDNF ELISA Kit PicoKine® (96 Tests). Quantitate Human CDNF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

人类

酶联免疫吸附测定

CDNF

Q49AH0

Expression system for standard: E.coli; Immunogen sequence: Q25-L187

There is no detectable cross-reactivity with other relevant proteins.

15.6 pg/ml - 1,000 pg/ml

<10 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA)

a单克隆抗体来自小鼠, a生物素多克隆抗体来自山羊

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cerebral dopamine neurotrophic factor

Cerebral dopamine neurotrophic factor; ARMET-like protein 1; Conserved dopamine neurotrophic factor; CDNF; ARMETL1

Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Secreted.

Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum.

Cerebral dopamine neurotrophic factor also known as ARMET-like protein 1 or is a protein that in humans that is encoded by the CDNF gene. CDNF and MANF are secreted proteins with 8 conserved cysteine residues, which predict a unique protein fold and define an evolutionarily conserved protein family. The human CDNF mRNA encodes a 187-amino acid protein with a predicted signal peptide and apparently no prosequence. The predicted secondary structure, like that of MANF, is dominated by alpha-helices. Human CDNF shares 59% amino acid identity with human MANF, and 49% and 46% identity with D. melanogaster and C. elegans Manf proteins, respectively. RT-PCR of mouse brain detected Cdnf transcripts in embryonic and postnatal stages, and in adult brain regions, including the striatum. In the midbrain, Cdnf mRNA was expressed from embryonic stages to the adult. High levels of Cdnf mRNA were observed in adult mouse heart, skeletal muscle, and testis. In agreement with the results from mouse, wide expression of CDNF transcripts was detected in human brain and nonneuronal tissues. Interestingly, levels of CDNF transcripts were relatively high in the corpus callosum and optic nerve, which are devoid of neuronal cell bodies and contain mostly axonal projections and oligodendrocytes. At postnatal stage P10 of the mouse brain, Cdnf staining was most intense in the hippocampus and thalamus. Cdnf signal was also detected in striatum and substantia nigra.

Growth And Development, Neurology Process, Neuroscience