产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human Angiogenin/ANG EZ-Set ELISA Kit (DIY Antibody Pairs)
目录 :
EZ0305
规格 :
For 5 plates, 96 wells each
价格 :
215美元
文章摘录数: 2
参考文献
Matter R, Abdelmaksoud A, Shams M, Bebawy E. Serum angiogenin level in sickle cell disease and beta thalassemia patients. Pediatr Hematol Oncol. 2014;31:50-6 pubmed 出版商
Yalcindag A, Gedik Oğuz Y, Yalçindağ F. The relationship between serum levels of angiogenin, bFGF, VEGF, and ocular involvement in patients with Behçet's disease. Graefes Arch Clin Exp Ophthalmol. 2013;251:1807-12 pubmed 出版商
产品信息
SKU号 :
EZ0305
状态 :
Enabled
名称 :
Human Angiogenin/ANG EZ-Set ELISA Kit (DIY Antibody Pairs)
目录名称 :
EZ Set ELISA Kits (Ab Pairs)
基因名称 :
ANG
价格 :
215美元
试验范围 :
78pg/毫升5000pg/毫升
componentsneeded :
Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects ANG in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
short description :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects ANG in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
description1 :
Product Features: . Your ideal low cost ELISA solution. EZ Set ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies. . Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
规格 :
For 5 plates, 96 wells each
UniProt数据库编号 :
P03950
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: Q25-P147
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<12pg/ml
kit components :
Catalog number Description Quantity Buffers EZ0305-CA Rabbit anti- human Angiogenin/ANG polyclonal antibody(Capture Antibody) 500 uL, 0.4mg/mL(recommended dilution 1:100) 0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol EZ0305-DA Biotinylated goat anti- human Angiogenin/ANG polyclonal antibody(Detection Antibody) 500 uL, 8.3ug/mL(recommended dilution 1:100) 0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol EZ0305-ST Lyophilized recombinant human Angiogenin/ANG standard 10ng/tubex2 AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 500ul(recommended dilution 1:100)
样品类型 :
cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human Angiogenin/ANG EZ Set ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 312pg/毫升, 156pg/毫升, 78pg/毫升
背景 :
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
competitor equivalent skus :
sc 8357
研究种类 :
Actin Binding Proteins, Actin Etc, Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton, Cytoskeleton / Ecm, Dna / Rna, Epigenetics And Nuclear Signaling, Hypoxia, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Response To Hypoxia, Rna Processing, Signal Transduction, Translation
同义词 :
Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;
基因全称 :
Angiogenin
分子量 :
16550分子量
protein function :
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
subcellular localization :
Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.
组织特异性 :
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
蛋白名 :
Angiogenin
sequence similarities :
Belongs to the pancreatic ribonuclease family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 312 672 1663 635 1856 3489 Standard deviation 9.4 23.52 54.9 45.72 165.2 300 CV(%) 3 3.5 3.3 7.2 8.9 8.6
样品数据 :
Concentration(pg/ml) 0.0 78 156 313 625 1250 2500 5000 O.D. 0.047 0.116 0.169 0.265 0.506 0.927 1.534 2.375
tmb incubation time :
15-20min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
6/11/19 6:28
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits