产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
人类BDNF EZ套酶联免疫吸附试剂盒(DIY抗体配对)
目录 :
EZ0307
规格 :
For 5 plates, 96 wells each, reagents only, plates are not included
价格 :
220美元
文章摘录数: 1
参考文献
Koeva Y, Sivkov S, Akabaliev V, Ivanova R, Deneva T, Grozlekova L, et al. Brain-derived neurotrophic factor and its serum levels in schizophrenic patients. Folia Med (Plovdiv). 2014;56:20-3 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EZ0307 图像 1
Human BDNF EZ Set ELISA Kit standard curve
产品信息
SKU号 :
EZ0307
状态 :
Enabled
名称 :
人类BDNF EZ套酶联免疫吸附试剂盒(DIY抗体配对)
目录名称 :
EZ Set ELISA Kits (Ab Pairs)
基因名称 :
BDNF
价格 :
220美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BDNF in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
short description :
A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BDNF in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.
规格 :
For 5 plates, 96 wells each, reagents only, plates are not included
UniProt数据库编号 :
P23560
宿主 :
No
免疫原 :
Expression system for standard: sf21; Immunogen sequence: H129-R247
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<15pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
人类BDNF EZ套酶联免疫吸附试剂盒标准曲线
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
背景 :
Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
同义词 :
Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF;
基因全称 :
脑源性神经营养因子
分子量 :
27818分子量
protein function :
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
subcellular localization :
Secreted.
组织特异性 :
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
蛋白名 :
脑源性神经营养因子
sequence similarities :
Belongs to the NGF-beta family.
样品数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.074 0.101 0.160 0.287 0.563 1.251 2.116
tmb incubation time :
15-25min
更新 :
5/9/19 5:52
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits