ELISA/assay

大鼠BMP-2 EZ套酶联免疫吸附试剂盒(DIY抗体配对)

EZ0312

For 5 plates, 96 wells each, reagents only, plates are not included

255美元

Enabled

大鼠BMP-2 EZ套酶联免疫吸附试剂盒(DIY抗体配对)

EZ Set ELISA Kits (Ab Pairs)

骨形态发生蛋白2

255美元

31.2pg/毫升2000pg/毫升

Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-2 in Rat samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-2 in Rat samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.

Product Features: . Your ideal low cost ELISA solution. EZ Set ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies. . Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

For 5 plates, 96 wells each, reagents only, plates are not included

P49001

No

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<2pg/ml

10ng/tube

bone tissue, cell culture supernates and serum.

酶联免疫吸附测定

大鼠

Rat BMP-2 EZ Set ELISA Kit standard curve Rat BMP-2 EZ Set ELISA Kit standard curve Rat BMP-2 EZ Set ELISA Kit standard curveRat BMP-2 EZ Set ELISA Kit standard curve

2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升

Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;

骨形态发生蛋白2

44383分子量

Induces cartilage and bone formation.

Secreted .

Expressed in femur, calvaria, trachea, lung and ovary.

骨形态发生蛋白2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 285 742 1197 306 834 1358 Standard deviation 12.25 37.84 51.47 16.21 45.87 55.67 CV(%) 4.3 5.1 4.3 5.3 5.5 4.1

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.489

15-25min

单克隆抗体来自小鼠多克隆抗体来自山羊

5/9/19 5:52